Cov kws tshawb fawb tau siv cov tshiab high-throughput ruaj khov isotope probing (HT-SIP) pipeline thiab metagenomics kom tau txais thawj zaug saib ntawm cov nquag microbiome nyob ib puag ncig cov nroj tsuag muaj txiaj ntsig symbiont, arbuscular mycorrhizal fungi (AMF). Credit: Lawrence Livermore National Laboratory
Txuas tus kheej ntawm cov tsiaj qus microbes nrog lawv lub cev zoo thiab kev ua haujlwm ib puag ncig yog lub hom phiaj tseem ceeb rau ib puag ncig microbiologists. Ntawm cov tswv yim uas siv zog rau lub hom phiaj no, Stable Isotope Probing-SIP-yog suav tias yog qhov zoo tshaj plaws rau kev kawm cov kab mob nquag hauv cov chaw ntuj.
Lawrence Livermore National Laboratory (LLNL) cov kws tshawb fawb tau tsim cov txheej txheem tshiab-siab-throughput SIP-uas automates ob peb kauj ruam nyob rau hauv tus txheej txheem ntawm ruaj khov isotope probing, tso cai rau kev tshawb fawb ntawm microbial kev ua ntawm cov kab mob nyob rau hauv cov kev mob tshwm sim tiag tiag, tsis tas yuav tsum tau rau kev kuaj culturing.
Hauv SIP, active microbes raug txheeb xyuas los ntawm kev koom ua ke ntawm cov isotopes ruaj khov rau hauv lawv cov biomass. Nws yog ib txoj hauv kev muaj zog tshaj plaws hauv microbial ecology vim nws tuaj yeem txheeb xyuas cov kab mob nquag thiab lawv lub cev zoo (substrate siv, cellular biochemistry, metabolism, kev loj hlob, kev tuag) hauv cov zej zog nyuaj nyob rau hauv ib txwm muaj.
Feem ntau, txoj kev SIP yuav tsum muaj kev ua haujlwm ntau ntawm tes thiab tsuas yog tso cai rau qee tus qauv. Tab sis cov txheej txheem LLNL tshiab yuav tsum muaj ib-rau ntawm tus nqi ntawm kev ua haujlwm ntawm tes piv rau phau ntawv SIP thiab tso cai rau 16 cov qauv ua tiav ib txhij.
"Peb txoj hauv kev ib nrab tsis siv neeg txo qis lub sijhawm ua haujlwm thiab txhim kho kev tsim tawm los ntawm kev tsom mus rau cov kauj ruam ua haujlwm hnyav tshaj plaws ntawm SIP," LLNL tus kws tshawb fawb Erin Nuccio, thiab tus thawj coj ntawm daim ntawv uas tau tshwm sim hauv phau ntawv xov xwm Microbiome. "Tam sim no peb tau siv txoj hauv kev no los ua ntau dua ib txhiab tus qauv, suav nrog qee qhov los ntawm cov av tsis tau kawm txog cov microhabitats."
Ib qho microhabitat yog cov av tam sim ntawd nyob ib puag ncig cov ntaub so ntswg ntawm mycorrhizae - ib hom fungi uas tsim kev sib raug zoo nrog 72% ntawm tag nrho cov nroj tsuag hauv av. Hauv kev pauv rau cov nroj tsuag carbon, cov kab mob (arbuscular mycorrhizal fungi) muab nws cov tswv nrog cov peev txheej tseem ceeb xws li nitrogen, phosphorus thiab dej.
Hauv cov ntaub ntawv pov thawj-ntawm txoj kev tshawb fawb no, cov kws sau ntawv tau qhia "cov khoom noj web" ntawm kev sib cuam tshuam los ntawm mycorrhizal fungi hauv av.
"Peb xav tias qhov no yog txoj hauv kev tseem ceeb rau yuav ua li cas cov nroj tsuag carbon tau dav dav faib rau hauv av. Av tuav lub pas dej loj tshaj plaws ntawm kev nquag caij tsheb kauj vab cov pa roj carbon monoxide, "said co-corresponding author Jennifer Pett-Ridge, uas yog LLNL project tus thawj coj thiab tus thawj coj ntawm Lub Tsev Haujlwm Saib Xyuas Lub Zog Lub Chaw Haujlwm Saib Xyuas Kev Tshawb Fawb "Microbes Persist" Soil Microbiome Scientific Focus Area . "Peb tau ua raws qee qhov me me ntawm DNA, txiav txim siab cov kab mob nquag thiab tom qab ntawd rov tsim kho lawv cov genomes thiab muaj peev xwm cuam tshuam."
Lwm tus kws sau ntawv LLNL suav nrog Steven Blazewicz, Marissa Lafler, Ashley Campbell, Jeffrey Kimbrel, Jessica Wollard, Rachel Hestrin thiab cov kws tshawb fawb los ntawm Lawrence Berkeley National Laboratory, DOE Joint Genome Institute thiab University of California, Berkeley.